Researcher Page
Maria de La Salette de Freitas F. Hipólito Reis D. Rodrigues (PhD)
Associate Professor with Aggregation (FFUP)
Address: REQUIMTE, Department of Chemistry, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal

Phone: +351 220428672, Fax: +351 226093390


GABAI Unit Coordination: Biophysics and Biomembranes

Research Areas:



Drug-membrane interactions, biomimetic membrane models, liposome, fluorimetry, nanopharmaceutics


- Use of biomimetic membrane models (micelles and liposomes) to study the effect of drugs on biological membranes trying to establish a relationship between this effect and their activity/mechanism of action. The techniques applied to study drug/membrane interactions are derivative spectroscopy; fluorescence anisotropy and quenching; differential scanning calorimetry (DSC); potentiometry and light scattering.

- Use of proteoliposomes to evaluate the membrane lipid composition on modulation of drugs interaction with membrane proteins.

- Nanopharmaceutics (liposomes and nanotubes) and drug delivery systems.




- Pinheiro M, Arede M, Giner-Casares JJ, Nunes C, Caio JM, Moiteiro C, Lucio M, Camacho L, Reis S. Effects of a novel antimycobacterial compound on the biophysical properties of a pulmonary surfactant model membrane. Int J Pharm, 2013. 450(1-2): p. 268-77. [DOI:10.1016/j.ijpharm.2013.03.062S0378-5173(13)00297-4 [pii]]

- Pinheiro M, Nunes C, Caio JM, Moiteiro C, Lucio M, Brezesinski G, Reis S. The influence of rifabutin on human and bacterial membrane models: implications for its mechanism of action. J Phys Chem B, 2013. 117(20): p. 6187-93. [DOI: 10.1021/jp403073v]

- Pereira-Leite, C., C. Carneiro, J.X. Soares, C. Afonso, C. Nunes, M. Lucio, and S. Reis, Biophysical characterization of the drug-membrane interactions: The case of propranolol and acebutolol. Eur J Pharm Biopharm, 2013. [DOI:pii: S0939-6411(12)00421-3. 10.1016/j.ejpb.2012.12.005]

- Pinheiro, M., M. Arede, C. Nunes, J.M. Caio, C. Moiteiro, M. Lucio, and S. Reis, Differential interactions of rifabutin with human and bacterial membranes: implication for its therapeutic and toxic effects. J Med Chem, 2013. 56(2): p. 417-26. [DOI: 10.1021/jm301116j]

- Neves, A.R., M. Lucio, S. Martins, J.L. Lima, and S. Reis, Novel resveratrol nanodelivery systems based on lipid nanoparticles to enhance its oral bioavailability. Int J Nanomedicine, 2013. 8: p. 177-87. [DOI: 10.2147/IJN.S37840]

- Pinheiro M, Giner-Casares JJ, Lucio M, Caio JM, Moiteiro C, Lima JLFC, Reis S, Camacho L. Interplay of mycolic acids, antimycobacterial compounds and pulmonary surfactant membrane: A biophysical approach to disease. Biochimica Et Biophysica Acta-Biomembranes, 2013. 1828(2): p. 896-905. [DOI: 10.1016/j.bbamem.2012.09.015]

- Pereira-Leite C, Nunes C, Lima JLFC, Reis S, Lucio M. Interaction of Celecoxib with Membranes: The Role of Membrane Biophysics on its Therapeutic and Toxic Effects. J Phys Chem B, 2012. 116(46): p.13608-13617. [DOI: 10.1021/jp304037v]

- Pinheiro, M., M. Lucio, S. Reis, J.L. Lima, J.M. Caio, C. Moiteiro, M.T. Martin-Romero, L. Camacho, and J.J. Giner-Casares, Molecular interaction of rifabutin on model lung surfactant monolayers. J Phys Chem B, 2012. 116(38): p. 11635-45. [DOI: 10.1021/jp303725j]

- Neves, A.R., M. Lucio, J.L. Lima, and S. Reis, Resveratrol in medicinal chemistry: a critical review of its pharmacokinetics, drug-delivery, and membrane interactions. Curr Med Chem, 2012. 19(11): p. 1663-81. [DOI: CMC-EPUB-20120117-009[pii]]

- Nunes, C., C.T. Sousa, M.P. Proenca, D.C. Leitao, J.L. Lima, S. Reis, J.P. Araujo, and M. Lucio, pH sensitive silica nanotubes as rationally designed vehicles for NSAIDs delivery. Colloids and Surfaces B-Biointerfaces, 2012. 94: p. 288-295. [DOI: 10.1016/j.colsurfb.2012.02.003]

- Cardoso, A.M., S. Trabulo, A.L. Cardoso, A. Lorents, C.M. Morais, P. Gomes, C. Nunes, M. Lucio, S. Reis, K. Padari, M. Pooga, M.C. Pedroso de Lima, and A.S. Jurado, S4(13)-PV cell-penetrating peptide induces physical and morphological changes in membrane-mimetic lipid systems and cell membranes: Implications for cell internalization. Biochim Biophys Acta, 2012. 1818(3): p. 877-88. [DOI: 10.1016/j.bbamem.2011.12.022]

- Pinheiro, M., M. Lúcio, J.L. Lima, and S. Reis, Liposomes as drug delivery systems for the treatment of TB. Nanomedicine (Lond), 2011. 6(8): p. 1413-1428. [DOI: 10.2217/nnm.11.122]

- Nunes, C., G. Brezesinski, C. Pereira-Leite, J.L. Lima, S. Reis, and M. Lúcio, NSAIDs interactions with membranes: a biophysical approach. Langmuir, 2011. 27(17): p. 10847-10858. [DOI: 10.1021/la201600y]

- Nunes, C., G. Brezesinski, D. Lopes, J.L. Lima, S. Reis, and M. Lúcio, Lipid-drug interaction: biophysical effects of tolmetin on membrane mimetic systems of different dimensionality. J Phys Chem B, 2011. 115(43): p. 12615-12623. [DOI: 10.1021/jp206013z]

- Nunes, C., G. Brezesinski, J.L.F.C. Lima, S. Reis, and M. Lúcio, Effects of non-steroidal anti-inflammatory drugs on the structure of lipid bilayers: Therapeutical aspects. Soft matter, 2011. 7(6): p. 3002-3010. [DOI:10.1039/c0sm00686f]

- Nunes, C., G. Brezesinski, J.L. Lima, S. Reis, and M. Lúcio, Synchrotron SAXS and WAXS study of the interactions of NSAIDs with lipid membranes. J Phys Chem B, 2011. 115(24): p. 8024-8032. [DOI: 10.1021/jp2025158]

- Monteiro, J.P., A.F. Martins, M. Lúcio, S. Reis, T.J. Pinheiro, C.F. Geraldes, P.J. Oliveira, and A.S. Jurado, Nimesulide interaction with membrane model systems: are membrane physical effects involved in nimesulide mitochondrial toxicity? Toxicol In Vitro, 2011. 25(6): p. 1215-1223. [DOI: 10.1016/j.tiv.2011.05.014]

- Monteiro, J.P., A.F. Martins, M. Lúcio, S. Reis, C.F. Geraldes, P.J. Oliveira, and A.S. Jurado, Interaction of carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP) with lipid membrane systems: a biophysical approach with relevance to mitochondrial uncoupling. J Bioenerg Biomembr, 2011. 43(3): p. 287-298. [DOI: 10.1007/s10863-011-9359-2]

- Gaspar, D., M. Lúcio, S. Rocha, J.L. Lima, and S. Reis, Changes in PLA(2) activity after interacting with anti-inflammatory drugs and model membranes: evidence for the involvement of tryptophan residues. Chem Phys Lipids, 2011. 164(4): p. 292-299. [DOI: 10.1016/j.chemphyslip.2011.03.003]

- Segundo, M.A., L.M. Magalhaes, J.P. Ribeiro, M. Lúcio, and S. Reis, Determination of the scavenging capacity against reactive nitrogen species by automatic flow injection-based methodologies. Methods Mol Biol, 2010. 704: p. 91-104. [DOI:10.1007/978-1-61737-964-2_8]

- Magalhães, L.M., C. Nunes, M. Lúcio, M.A. Segundo, S. Reis, and J.L. Lima, High-throughput microplate assay for the determination of drug partition coefficients. Nat Protoc, 2010. 5(11): p. 1823-1830. [DOI:10.1038/nprot.2010.137]

- Lúcio, M., J.L. Lima, and S. Reis, Drug-membrane interactions: significance for medicinal chemistry. Curr Med Chem, 2010. 17(17): p. 1795-809. [DOI: BSP/CMC/E-Pub/ 107 [pii]]

- Gaspar, D., M. Lúcio, K. Wagner, G. Brezesinski, S. Rocha, J.L. Costa Lima, and S. Reis, A biophysical approach to phospholipase A2 activity and inhibition by anti-inflammatory drugs. Biophys Chem, 2010. 152(1-3): p.109-17. [DOI:10.1016/j.bpc.2010.08.006]

- Brittes, J., M. Lúcio, C. Nunes, J.L. Lima, and S. Reis, Effects of resveratrol on membrane biophysical properties: relevance for its pharmacological effects. Chem Phys Lipids, 2010. 163(8): p. 747-54. [DOI: 10.1016/j.chemphyslip.2010.07.004]

- Magalhães, L.M., M. Lúcio, M.A. Segundo, S. Reis, and J.L. Lima, Automatic flow injection based methodologies for determination of scavenging capacity against biologically relevant reactive species of oxygen and nitrogen. Talanta, 2009. 78(4-5): p. 1219-26. [DOI: 10.1016/j.talanta.2009.02.006]

- Lucio, M., C. Nunes, D. Gaspar, K. Golebska, M. Wisniewski, J.L.F.C. Lima, G. Brezesinski, and S. Reis, Effect of anti-inflammatory drugs in phosphatidylcholine membranes: A fluorescence and calorimetric study. Chemical Physics Letters, 2009. 471(4-6): p. 300-309. [DOI:10.1016/j.cplett.2009.02.047]

- Lúcio, M., C. Nunes, D. Gaspar, H. Ferreira, J.L.F.C. Lima, and S. Reis, Antioxidant activity of vitamin E and Trolox: Understanding of the factors that govern lipid peroxidation studies in Vitro Food Biophysics 2009. 4 (4): p. 312-320. [DOI: 10.1007/s11483-009-9129-4]

- Araújo, A.R., D. Gaspar, M. Lúcio, S. Reis, M.L. Saraiva, and J.L. Lima, Sequential injection system for phospholipase A2 activity evaluation: studies on liposomes using an environment-sensitive fluorescent probe. Talanta, 2009. 79(4): p. 1125-9.[DOI: 10.1016/j.talanta.2009.03.029]

- Sousa, C., C. Nunes, M. Lúcio, H. Ferreira, J.L. Lima, J. Tavares, A. Cordeiro-da-Silva, and S. Reis, Effect of nonsteroidal anti-inflammatory drugs on the cellular membrane fluidity. J Pharm Sci, 2008. 97(8): p. 3195-3206. [DOI:10.1002/jps.21218]

- Santos, F., L. Teixeira, M. Lúcio, H. Ferreira, D. Gaspar, J.L. Lima, and S. Reis, Interactions of sulindac and its metabolites with phospholipid membranes: an explanation for the peroxidation protective effect of the bioactive metabolite. Free Radic Res, 2008. 42(7): p. 639-650. [DOI: 10.1080/10715760802270326]

- Rocha, S., M. Lúcio, M.C. Pereira, S. Reis, and G. Brezesinski, The conformation of fusogenic B18 peptide in surfactant solutions. J Pept Sci, 2008. 14(4): p. 436-441. [DOI: 10.1002/psc.935]

- Nunes, C., C. Sousa, H. Ferreira, M. Lúcio, J.L. Lima, J. Tavares, A. Cordeiro-da-Silva, and S. Reis, Substituted phenols as pollutants that affect membrane fluidity. J Environ Biol, 2008. 29(5): p. 733-738. [N/A]

- Lúcio, M., H. Ferreira, J.L. Lima, and S. Reis, Use of liposomes as membrane models to evaluate the contribution of drug-membrane interactions to antioxidant properties of etodolac. Redox Rep, 2008. 13(5): p. 225-236. [DOI: 10.1179/135100008X308939]

- Lúcio, M., F. Bringezu, S. Reis, J.L. Lima, and G. Brezesinski, Binding of nonsteroidal anti-inflammatory drugs to DPPC: structure and thermodynamic aspects. Langmuir, 2008. 24(8): p. 4132-4139. [DOI: 10.1021/la703584s]

- Lúcio, M., H. Ferreira, J.L. Lima, and S. Reis, Use of liposomes to evaluate the role of membrane interactions on antioxidant activity. Anal Chim Acta, 2007. 597(1): p. 163-170. [DOI:10.1016/j.aca.2007.06.039]

- Lúcio, M., H. Ferreira, J.L. Lima, and S. Reis, Interactions between oxicams and membrane bilayers: an explanation for their different COX selectivity. Med Chem, 2006. 2(5): p. 447-456. [PubMed ID: 17017983]

- Ferreira, H., M. Lúcio, J.L. Lima, C. Matos, and S. Reis, Effects of diclofenac on EPC liposome membrane properties. Anal Bioanal Chem, 2005. 382(5): p. 1256-1264. [DOI: 10.1007/s00216-005-3251-z]

- Ferreira, H., M. Lucio, J.L. Lima, C. Matos, and S. Reis, Interaction of clonixin with EPC liposomes used as membrane models. J Pharm Sci, 2005. 94(6): p. 1277-87. [DOI: 10.1002/jps.20351]

- Ferreira, H., M. Lúcio, J.L. Lima, A. Cordeiro-da-Silva, J. Tavares, and S. Reis, Effect of anti-inflammatory drugs on splenocyte membrane fluidity. Anal Biochem, 2005. 339(1): p. 144-149. [DOI: 10.1016/j.ab.2004.12.023]

- Lúcio, M., H. Ferreira, J.L.F.C. Lima, C. Matos, B. De Castro, and S. Reis, Influence of some anti-inflammatory drugs in membrane fluidity studied by fluorescence anisotropy measurements Physical Chemistry Chemical Physics 2004. 6 (7): p. 1493-1498. [DOI: 10.1039/B314551B]

- Ferreira, H., M. Lúcio, B. de Castro, P. Gameiro, J.L. Lima, and S. Reis, Partition and location of nimesulide in EPC liposomes: a spectrophotometric and fluorescence study. Anal Bioanal Chem, 2003. 377(2): p. 293-298. [DOI: 10.1007/s00216-003-2089-5]



- Lúcio, M.; Lima, J. L. F. C. and Reis, S., Drug-membrane interactions: molecular mechanisms underlying therapeutic and toxic effects of drugs. Ideas in Chemistry and Molecular Sciences: Where chemistry meets life, 2010, Wiley-VCH Verlag,Weinheim. [DOI: 10.1002/9783527630516.ch8]

- Reis, S., M. Lúcio, M. Segundo, and J.L. Lima, Use of liposomes to evaluate the role of membrane interactions on antioxidant activity. Methods Mol Biol, 2009. 606: p. 167-88. [DOI: 10.1007/978-1-60761-447-0_13]




- Ana Catarina Rodrigues Alves - "Design, synthesis and characterization of innovative nanosystems for targeted delivery of anticancer drugs"

- Ana Rute de Azevedo Pina Neves - "A membrane biophysical approach to the therapeutic effects of resveratrol and design of novel nutraceutical nanoformulations"

- Marina Barroso Pereira Pinheiro - "Interactions of Rifabutin and Novel Analogs for Tuberculosis with Membranes: Implications for Mechanism of Action and Drug Development"



- Cláudia Daniela Oliveira de Lacerda Nunes Pinho - "New approaches to the study of the therapeutic action and side effects of NSAIDs comprising biophysical aspects of the drug-membrane interaction" (2011)

- Diana Maria Diez Gaspar - “Avaliação do efeito de fármacos na actividade de proteínas membranares. Utilização de proteolipossomas como sistemas miméticos de biomembranas” (2010)

- Luís Miguel Andrade de Magalhães (co-supervision) - “Desenvolvimento de métodos analíticos automáticos baseados em técnicas de fluxo contínuo para avaliação de propriedades antioxidantes em produtos farmacêuticos e em alimentos” (2007)

- David de Andrade Sousa Costa (co-supervision) - “Desenvolvimento e aplicação de metodologias in vitro para avaliação da actividade anti‑inflamatória e anti-oxidante de fármacos anti-inflamatórios não esteróides (NSAIDs)” (2007)

- Marlene Susana Dionísio Lúcio - “Avaliação e quantificação das interacções de fármacos com agregados de sais biliares. Sua influência na biodisponibilidade desses fármacos” (2006)

- Helena Susana da Costa Machado Ferreira - “Utilização de lipossomas como modelos de biomembranas na avaliação e quantificação da actividade de anti-inflamatórios” (2006)

- Carla Manuela Soares de Matos - “Encapsulação de fármacos em lipossomas: Efeito nas propriedades físico-químicas” (2001)

- Carla Maria Sanfins Guimarães - “Distribuição de fármacos em sistemas anfipáticos mistos: aplicação ao estudo da dissolução e transporte de bloqueadores-b” (2000)

- Christophe Raymond André Marc Siquet - “Síntese de compostos fenólicos: estudo da relação estrutura-propriedades físico-químicas-actividade antioxidante”



- Evaluation of novel nonsteroidal anti-inflammatory drugs on the biophysical properties of the membrane studied 2D model systems. CALIPSO founded program: Sirius-2 beamline at Soleil Synchrotron, Saint-Aubin, France.

- Antibody-directed liposomes for Alzheimer`s disease. PTDC/QUI-BIQ/118076/2010, funded by Fundação para a Ciência e Tecnologia (Fundation for Science and Technology), Portugal.

- Development of azacalix(hetero)arene transporters for mediated anionic flux across membranes: a novel approach for studying ionic passive diffusion. PTDC/QUI-QUI/101022/2008, funded by Fundação para a Ciência e Tecnologia (Fundation for Science and Technology), Portugal.


GABAI-Grupo de Análises Bioquímicas, Ambientais e Industriais, 2013

Dept of Chemical Sciences | Faculty of Pharmacy of University of Porto | Rua de Jorge Viterbo de Ferreira, 228, 4050 – 313 Porto, Portugal | Tel. +351 220428500 | Fax. +351 226093483