GABAI

	Ana Rute de Azevedo Pina Neves (PhD)
	PostDoc Researcher (REQUIMTE)
	Address: REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal Phone: +351 220428672, Fax: +351 226093390 Email: ananeves@ff.up.pt

Research Areas:

Biophysics, Biochemistry, and Nanotechnology

Keywords:

Drug delivery, lipid nanoparticles (SLN, NLC), liposomes, biomimetic systems, drug-membrane interactions

Topics:

- Biophysical and biochemical approaches to study the interaction of drugs with biomembranes (using liposomes and monolayers as biomimetic systems) in order to explain therapeutic properties.

- Development of nanodelivery systems with specific functionalization for drug delivery and controlled drug release.

PhD in Pharmaceutical Sciences: "A membrane biophysical approach to the therapeutic effects of resveratrol and design of novel nutraceutical nanoformulations."

Publications:

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PEER-REVIEWED PAPERS:

17. Neves A. R., Queiroz J. F., Lima S. A. C., Reis S., ApoE functionalization of solid lipid nanoparticles enhances brain drug delivery: uptake mechanism and transport pathways. Bioconjugate Chem. 2017, 28, 995-1004. [DOI: 10.1021/acs.bioconjchem.6b00705]

16. Loureiro J. A., Andrade S., Duarte A., Neves A. R., Queiroz J. F., Nunes C., Sevin E., Fenart L., Gosselet F., Coelho M. A. N., Pereira M. C., Resveratrol and Grape Extract-Loaded Solid Lipid Nanoparticles for the Treatment of Alzheimer’s disease. Molecules 2017, 22, 277. [DOI: 10.3390/molecules22020277]

15. Neves A. R., Nunes C., Amenitsch H., Reis S., Resveratrol interaction with lipid bilayers: a synchrotron X-ray scattering study. Langmuir 2016, 32, 12914-12922. [DOI: 10.1021/acs.langmuir.6b03591]

14. Frias I., Neves A. R., Pinheiro M., Reis S., Design, development and characterization of lipid nanocarriers-based epigallocatechin gallate delivery system for preventive and therapeutic supplementation. Drug Des Devel Ther. 2016, 10, 3519-3528. [DOI: 10.2147/DDDT.S109589]

13. Barbosa J. P., Neves A. R., Silva A. M., Barbosa M. A., Reis S., Santos S. G., Nanostructured Lipid Carriers Loaded with Resveratrol Modulate Human Primary Dendritic Cells. Int. J. Nanomedicine 2016, 11, 3501–3516. [DOI: 10.2147/IJN.S108694]

12. Neves A. R., Queiroz J. F., Reis S., Brain-targeted delivery of resveratrol using solid lipid nanoparticles functionalized with apolipoprotein E. J. Nanobiotechnol. 2016, 14 (27), 1-11. [DOI: 10.1186/s12951-016-0177-x]

11. Neves A. R., Martins S., Segundo M. A., Reis S., Nanoscale delivery of resveratrol towards enhancement of supplements and nutraceuticals. Nutrients 2016, 8, 131. [DOI: 10.3390/nu8030131]

10. Neves A. R., Nunes C., Amenitsch H., Reis S., Effects of resveratrol on the structure and fluidity of lipid bilayers: a membrane biophysical study. Soft Matter 2016, 12, 2118 – 2126. [DOI: 10.1039/C5SM02905H]

9. Neves A. R., Queiroz J. F., Lima S. A. C., Figueiredo F., Fernandes R., Reis S., Cellular uptake and transcytosis of lipid-based nanoparticles across the intestinal barrier: relevance for oral drug delivery. J. Colloid Interface Sci. 2016, 463, 258–265. [DOI: 10.1016/j.jcis.2015.10.057]

8. Neves A. R., Nunes C., Reis S., Resveratrol induces ordered domains formation in biomembranes: Implication for its pleiotropic action. Biochimica et Biophysica Acta 2016, 1858, 12–18. [DOI: 10.1016/j.bbamem.2015.10.005]

7. Lopes–de-Araújo J., Neves A. R., Gouveia V. M., Moura C. C., Nunes C. and Reis S., Oxaprozin-loaded lipid nanoparticles towards overcoming NSAIDs side-effects. Pharm. Res. 2016, 33(2), 301-14. [DOI: 10.1007/s11095-015-1788-x]

6. Neves A. R., Queiroz J. F., Weksler B., Romero I. A., Couraud PO., Reis S., Solid lipid nanoparticles as a vehicle for brain-targeted drug delivery: two new strategies of functionalization with apolipoprotein E. Nanotechnology 2015, 26, 495103-13. [DOI: 10.1088/0957-4484/26/49/495103]

5. Neves A. R., Nunes C., Reis S., New insights on the biophysical interaction of resveratrol with biomembrane models: relevance for its biological effects. J. Phys. Chem. B. 2015, 119(35), 11664-72. [DOI: 10.1021/acs.jpcb.5b05419]

4. Neves A. R., Reis S., Segundo M. A., Development and validation of a HPLC method using a monolithic column for quantification of trans-resveratrol in lipid nanoparticles for intestinal permeability studies. J. Agric. Food Chem., 2015, 63, 3114−3120. [DOI: 10.1021/acs.jafc.5b00390]

3. Neves A. R., Lúcio M., Martins S., Lima J. L. F. C., Reis S., Novel resveratrol nanodelivery systems based on lipid nanoparticles to enhance its oral bioavailability. Int. J. Nanomedicine, 2013, 8:177-187. [DOI: 10.2147/IJN.S37840] [ Highly Cited Paper according to the Essential Science IndicatorsSM]

2. Neves A. R., Lúcio M., Lima J. L. F. C., Reis S., Resveratrol in medicinal chemistry: a critical review of its pharmacokinetics, drug-delivery, and membrane interactions. Curr. Med. Chem., 2012, 19(11):1663-81. [DOI: 10.2174/092986712799945085] [ Highly Cited Paper according to the Essential Science IndicatorsSM]

1. Neves A. R., Fernandes P. A., Ramos M. J., The accuracy of density functional theory in the description of cation-π and π-hydrogen bond interactions, J. Chem. Theory Comput. 2011, 7 (7), 2059-2067. [DOI: 10.1021/ct2001667]

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BOOK CHAPTERS:

4. Neves A. R. and Reis S., Importance and application of nanotechnology for improving the existing therapy, Book Series: Nanoparticles in the Life Sciences and Biomedicine, 2016, with the ISBN 978-981-4745-98-7 for hardcover and ISBN 978-981-4745-99-4 for eBook.

3. Neves A. R. and Reis S., Nanoparticles and new challenges on site-specific brain drug-delivery, Book Series: Nanoparticles in the Life Sciences and Biomedicine, 2016, with the ISBN 978-981-4745-98-7 for hardcover and ISBN 978-981-4745-99-4 for eBook.

2. Albuquerque J., Neves A. R. and Reis S., Theranostics: simultaneous treatment and diagnosis made possible by nanotechnology, Book Series: Nanoparticles in the Life Sciences and Biomedicine, 2016, with the ISBN 978-981-4745-98-7 for hardcover and ISBN 978-981-4745-99-4 for eBook.

1. Albuquerque J., Neves A. R. and Reis S., Wheat Germ: Protein Contents and Their Dietary Benefits, Book Series: Agricultural Research Updates, 2016, with the ISBN 978-1-63485-648-5.

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BOOK SERIES EDITOR:

1. Neves A. R. and Reis S., editors of the Book Series: Nanoparticles in the Life Sciences and Biomedicine, 2016, with the ISBN 978-981-4745-98-7 for hardcover and ISBN 978-981-4745-99-4 for eBook.

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INVITED ORAL COMUNICATIONS:

6. A. R. Neves, J. F. Queiroz, L. Van der Putten, and S. Reis, Recent advances on brain drug delivery for the treatment of neurodegenerative diseases, MAD-NANO16: Madeira International Conference on Emerging Trends and Future of Nanomaterials for Human Health, Funchal, Madeira Island, 2016.

5. A. R. Neves, F. M. Almeida, C. Nunes and S. Reis, Resveratrol modulates cell membranes: what’s the story?, Porto Polyphenols 2016 - 10th World Congress on Polyphenols Applications, Instituto Superior de Engenharia do Porto, 2016.

4. A. R. Neves, J. F. Queiroz, L. Van der Putten, and S. Reis, Improving Phytochemicals Brain Delivery using Powerful Nanotechnology, 5º Encontro de Biotecnologia Microbiana e Farmacêutica, Faculdade de Farmácia da Universidade do Porto, 2016.

3. A. R. Neves, S. A. Costa Lima and S. Reis, Challenges and advances in oral drug delivery using lipid-based nanoparticles, Pharmaceutica 2016 - 8th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems, Madrid, Spain, 2016.

2. A. R. Neves, J. F. Queiroz, and S. Reis, Solid lipid nanoparticles as a vehicle for brain-targeted delivery of resveratrol, Encontro organizado para a visita do investigador Norueguês (Dr. Gareth Griffiths), na Faculdade de Farmácia da Universidade do Porto, 2014.

1. A. R. Neves, M. Lúcio, S. Martins, J. L. F. C. Lima and S. Reis, Desenvolvimento de nanopartículas lipídicas contendo resveratrol: sua aplicação como nutracêutico, 1º Encontro de Biotecnologia Microbiana e Farmacêutica, Faculdade de Farmácia da Universidade do Porto, 2012.

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OTHER INTERNATIONAL ORAL COMUNICATIONS:

7. A. R. Neves, J. F. Queiroz, L. Van der Putten, and S. Reis, Development of Smart Nanoparticles for Bioactive Phytochemicals Brain Delivery, BioNanoMed 2016 - 7th International Congress Nanotechnology in Biology & Medicine, Krems, Austria, 2016.

6. A. R. Neves, C. Nunes and S. Reis, Interaction of resveratrol with lipid membranes: relevance for the therapeutic effects, XXI Encontro Galego-Portugués de Química, Pontevedra, Spain, 2015.

5. A. R. Neves, C. Nunes and S. Reis, A membrane biophysical approach to the anticarcinogenic effects of resveratrol, Resveratrol 2015 Regional Meeting, Dijon, Bourgogne, France, 2015.

4. A. R. Neves, J. F. Queiroz, S. Reis, Brain-targeted delivery of resveratrol using Apo E-functionalized solid lipid nanoparticles, Resveratrol 2014 – 3rd International Conference on Resveratrol and Health, Waikoloa Village, Big Island, Hawaii, EUA, 2014.

3. A. R. Neves, S. Martins, M. A. Segundo, J. L. F. C. Lima and S. Reis, Nanoscale delivery of resveratrol across the human intestinal caco-2 cell monolayers, XIX Encontro Galego-Portugués de Química, Vigo, Spain, 2013.

2. A. R. Neves, M. Lúcio, J. L. F. C. Lima and S. Reis, Encapsulation of resveratrol in lipid nanoparticles: formulation and characterization, 3PYCheM - 3rd Portuguese Young Chemists Meeting, Porto, 2012.

1. A. R. Neves, N. Rosa, M. Lúcio, J. L. F. C. Lima and S. Reis, Development and characterization of lipid nanoparticles loaded with resveratrol, XVII Encontro Galego-Portugués de Química, Pontevedra, Spain, 2011.

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POSTERS:

7. A. R. Neves, F. M. Almeida, C. Nunes and S. Reis, Unraveling the anticarcinogenic effects of resveratrol through a membrane biophysical approach, 5th International Iberian Biophysics Congress, Seminário de Vilar do Porto, 2016.

6. A. R. Neves, J. F. Queiroz, S. Reis, Brain-targeted delivery of resveratrol using Apo E-functionalized solid lipid nanoparticles, Resveratrol 2014 – 3rd International Conference on Resveratrol and Health, Waikoloa Village, Big Island, Hawaii, EUA, 2014.

5. A. R. Neves, J.L.F.C. Lima, S. Reis, M. Segundo, Development and validation of a reversed-phase HPLC method for the determination of resveratrol in lipid nanoparticles from cell culture media, Symposium of the Analytical Division of SPQ, Coimbra, Porto, 2014.

4. A. R. Neves, C. Nunes, J.L.F.C. Lima, S. Reis, Resveratrol interactions with biomembrane model systems, NovAlix 2013 - Biophysics in Drug Discovery, Strasbourg, France, 2013.

3. A. R. Neves, S.A.C. Lima, J.L.F.C. Lima and S. Reis, Nanoimaging of resveratrol-loaded lipid nanoparticles during Caco-2 cell uptake, BioImaging 2013 – 2ndInternational Symposium in Applied Bioimaging – From Molecule to Man, Biblioteca Almeida Garrett, Porto, 2013.

2. A.R. Neves, S. Martins, D. Ferreira, J. L. F. C. Lima and S. Reis, Nanoscale delivery of resveratrol by using lipid nanoparticles: a cell study approach, NanoPT 2013 – Nanoscience and Nanotechnology International Conference, Porto, 2013.

1. A.R. Neves, A. C. Alves, N. Rosa, A. Rentão, R. Amaral, S. Martins, D. Ferreira, J.L.F.C. Lima, S. Reis and M. Lúcio, Development of novel drug delivery systems based on lipid nanoparticles, RICI4 – 4th Iberian Meeting on Colloids and Interfaces, Porto, 2011.

Supervision:

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CO-SUPERVISION:

2016/2020 PhD Thesis of João Pedro Albuquerque e Costa: “From mouth to mammary glands: a nanotechnological application in dairy cow nutrition for targeted lysine delivery”, Doctoral Program in Animal Science, ICBAS.

2016/2018 Master Thesis of Ana Cristina Rafael Joyce Coutinho: “Design of nanodelivery systems for brain cancer treatment using genistein as a potent chemopreventive agent”, Master in Pharmaceutical Chemistry, FFUP.

2016/2017 Master Thesis of Rúben Gonçalo Rodrigues Pinheiro: “Development of quercetin brain delivery systems for the treatment of neurodegenerative diseases”, Integrated Master in Bioengineering, FEUP/ICBAS.

2015/2016 Master Seminar of Ana Cristina Rafael Joyce Coutinho: “Nanotechnology to Improve Bioactive Genistein Efficacy”, Master in Pharmaceutical Chemistry, FFUP.

2015/2016 Integrated Laboratory Course of Rúben Gonçalo Rodrigues Pinheiro: “Development of quercetin brain delivery systems for the treatment of neurodegenerative diseases”, Integrated Master in Bioengineering, FEUP/ICBAS.

2015/2016 Research Project I Course of Maria Francisca Mendes de Almeida: “Unraveling the mechanisms responsible for ulcerogenic properties of resveratrol: a membrane biophysical approach”, Integrated Master in Pharmaceutical Sciences, FFUP.

2014/2015 Master Thesis of Lizzie van der Putten: ”Development of nanodelivery systems for curcumin brain release: new insights for the treatment of Alzheimer’s Disease”, Master in Pharmaceutical Sciences of Utrecht University, Netherlands, ERASMUS Mundus Program.

2013/2014 Master Thesis of Joana Sofia Fontes de Queiroz: “Resveratrol loaded lipid nanoparticles for brain targeted delivery”, Integrated Master in Bioengineering, FEUP/ICBAS.

2012/2013 Research Project I Course of João Portugal Barbosa: “Resveratrol-loaded nanoparticles to modulate dendritic cells response to a pro-inflammatory environment”, Integrated Master in Pharmaceutical Sciences, FFUP.

Projects:

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PARTICIPATION AS MEMBER OF TEAM:

CELLS founded program - 2016021579: “Assessment of the mechanisms and triggers of the lamellar/inverse hexagonal phase transition in liposomes”, BL11 – NCD beamline at Cells-Alba Synchrotron, Barcelona, Spain, 2016.

CALIPSO founded program - 20141247: “Biophysical studies of antimicrobial drugs-membrane Interactions using 2D membrane mimetic models”, Sirius-2 beamline at Soleil Synchrotron, Saint-Aubin, Paris, France, 2014.

ELETTRA founded program - 20135320: “Evaluation of anti-tuberculosis drugs on the biophysical properties of the membrane studied 3D model systems”, Elettra Sincrotrone, Triestre, Itália, 2013.

CALIPSO founded program - 20130142: “Evaluation of novel nonsteroidal anti-inflammatory drugs on the biophysical properties of the membrane studied 2D model systems”, Sirius-2 beamline at Soleil Synchrotron, Saint-Aubin, Paris, France, 2013.

DOOR HASYLAB founded program - I-20110271 EC 2011-2012: “Biophysical studies of cell penetrating peptides (CPP) on 3D and 2D membrane model systems”, BW1 beamline at Doris III DESY HASYLAB Deutsches Elektronen-Synchrotron, Hamburg, Germany, 2012.

Additional Info:

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SCIENCE DISSEMINATION PAPERS:

- The power of nanopharmaceutics to cure deadly diseases, International Innovation – Disseminating Science, Research and Technology 2015, 185: 96-98.

http://edition.pagesuite-professional.co.uk/launch.aspx?eid=9d5071ec-31dd-449b-8508-5b3fe627912b&pnum=98

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CONFERENCE PROCEEDINGS:

- Sousa, S. F.; Cerqueira, N. M. F. S. A.; Perez, M. A. S.; Moreira, I. S.; Ribeiro, A. J. M.; Neves, A. R. A. P.; Ramos, M. J.; Fernandes, P. A.; Recent Advances on Computational Proteomics. Internacional Congress WASET - Conference on Bioinformatics, Computational and Systems Biology, Singapore, August 2009.

http://waset.org/publications/15475/recent-advances-on-computational-proteomics

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INVITED ORAL COMUNICATION DISSEMINATION :

- Ana Rute Neves, University of Porto, Portugal, Challenges and advances in oral drug delivery using lipid-based nanoparticles, 8th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems, March 07-09, 2016 Madrid, Spain.

http://novel-drugdelivery-systems.pharmaceuticalconferences.com/speaker/2016/ana-rute-neves-university-of-porto-portugal

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VIDEO ABSTRACT:

- Neves A. R., Lúcio M., Martins S., Lima J. L. F. C., Reis S., Novel resveratrol nanodelivery systems based on lipid nanoparticles to enhance its oral bioavailability, Int. J. Nanomedicine 2013, 8: 177-187. [Highly Cited Paper according to the Essential Science Indicators.]

https://www.youtube.com/watch?v=Sd4qHcHJz-w

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AUDIOSLIDES:

- Neves A. R., Queiroz J. F., Lima S. A. C., Figueiredo F., Fernandes R., Reis S., Cellular uptake and transcytosis of lipid-based nanoparticles across the intestinal barrier: relevance for oral drug delivery, J. Colloid Interface Sci 2016, 463: 258–265.

http://audioslides.elsevier.com/ViewerSmall.aspx?doi=10.1016/j.jcis.2015.10.057&Source=0&resumeTime=0&resumeSlideIndex=1&width=800&height=639

- Neves A. R., Nunes C., Reis S., Resveratrol induces ordered domains formation in biomembranes: Implication for its pleiotropic action, Biochimica et Biophysica Acta 2016, 1858: 12–18. [Key Scientific Article according to the Global Medical Discovery].

http://audioslides.elsevier.com/ViewerSmall.aspx?doi=10.1016/j.bbamem.2015.10.005&Source=0&resumeTime=0&resumeSlideIndex=0&width=800&height=639

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LABTALK IN NANOTECHWEB.ORG:

- Smart nanoparticles actively deliver to the brain, Nanotechnology 2015, 26: 495103-13.

http://nanotechweb.org/cws/article/lab/63568

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GLOBAL MEDICAL DISCOVERY (KEY SCIENTIFIC ARTICLES):

- Neves A. R., Lúcio M., Lima J. L., Reis S., Resveratrol in medicinal chemistry: a critical review of its pharmacokinetics, drug-delivery, and membrane interactions, Curr Med Chem 2012, 19(11): 1663-81.

https://globalmedicaldiscovery.com/key-scientific-articles/resveratrol-in-medicinal-chemistry-a-critical-review-of-its-pharmacokinetics-drug-delivery-and-membrane-interactions/

- Neves A. R., Nunes C., Reis S., Resveratrol induces ordered domains formation in biomembranes: Implication for its pleiotropic action, Biochim Biophys Acta. 2016, 1858(1): 12-8.

https://globalmedicaldiscovery.com/key-drug-discovery-articles/resveratrol-induces-ordered-domains-formation-biomembranes-implication-pleiotropic-action/

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ATLAS OF SCIENCE:

- Neves A. R., Martins S., Segundo M. A., Reis S., Design of supplements and nutraceuticals enriched in resveratrol: a promising natural compound, Nutrients 2016, 8(3): 131.

http://atlasofscience.org/design-of-supplements-and-nutraceuticals-enriched-in-resveratrol-a-promising-natural-compound/

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BIOWEBSPIN:

- Neves A. R., Queiroz J. F., Reis S., Smart nanoparticles for active brain drug delivery, Biowebspin Opportunities Portfolio 2015.

http://www.biowebspin.com/proposal-detail/697_115638_smart-nanoparticles-for-active/

- Neves A. R., Almeida F. M., Reis S., Design of novel nutraceutical nanoformulations of resveratrol for oral administration, Biowebspin Opportunities Portfolio 2015.

http://www.biowebspin.com/proposal-detail/698_115638_design-of-novel-nutraceutical/

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TWITTER:

- Neves A. R., Martins S., Segundo M. A., Reis S., Design of novel nutraceutical nanoformulations of resveratrol with health benefits similar to the red wine consumption. MDPI Twitter 2016.

https://twitter.com/Nutrients_MDPI/status/745074533934080000

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NUTRAINGREDIENTS | FOODNAVIGATOR:

- Ana Rute Neves, What’s new for an old molecule like resveratrol? 10th World Congress on Polyphenol Applications 2016, Journal of International Society of Antioxidants in Nutrition and Heath [DOI: 10.18143/JISANH_v3i4_1417]

http://www.nutraingredients.com/Research/What-s-new-for-an-old-molecule-like-resveratrol